Friday, December 25, 2009

Primer Melting Temp What Is The Lowest Reasonable Melting Temperature For PCR Primers?

What is the lowest reasonable melting temperature for PCR primers? - primer melting temp

... Taq polymerase used. Temp default extension of 72 degrees. I had a brain fart and ordered primers with a melting point of 40 degrees (if you're by the rule of 2 / 4) and I'm not sure if this is the annealing 30something and see what happens, or new primers, which longer have to.

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